@article{Dudikova_Suvorova_Nedashkivska_Sharova_Dronova_Vrynchanu_2018, title={Antibiofilm activity of aminopropanol derivatives against Pseudomonas aeruginosa}, url={https://pharmj.org.ua/index.php/journal/article/view/56}, DOI={10.32352/0367-3057.1.17.12}, abstractNote={<p>Bacterial biofilm, particularly formed by <em>Pseudomonas aeruginosa</em>, are a cause of severe chronic infectious diseases. Bacteria within a biofilm are phenotypically more resistant to antibiotics and the macroorganism immune system, making it an important virulence factor for many microbes.</p> <p>The aminopropanol derivatives with adamantyl (KVM-97) and N-alkylaryl radicals (KVM-194, KVM-204, KVM-261, and KVM-262) were used as study object. The aim of this study was to investigate the antibiofilm activity of compounds on biofilm formation and on mature biofilm of <em>P. aeruginosa</em>. The effects of the aminopropanol derivatives on the biofilm mass were evaluated by using crystal violet assay. Ciprofloxacin, meropenem, ceftazidime, gentamicin were used as reference substances.</p> <p>Reported results demonstrate that all compounds displayed antibiofilm activity at the tested concentrations. Remarkable reduction in biofilm formation of <em>P.&nbsp;aeruginosa</em> was found after treatment with KVM-97, KVM-261 and KVM-262 in high concentration (5× MIC), biofilm inhibition activity were 84.3%, 90.5% and 83.3% respectively.</p> <p>After a treatment with KVM-204 at 250 μg/ml (5× MIC) 76.6% of the preformed 24-hr biofilms were destroyed. Furthermore, compounds KVM-97, KVM-194, and KVM-261 in both concentrations showed potent antibiofilm activity against the <em>P.&nbsp;aeruginosa</em>, inhibition activity values being between 56.7 and 65.7%.</p> <p>All tested compounds in dose-dependent manner exhibited pronounced inhibition activity against mature 5-days <em>P.</em>&nbsp;<em>аeruginosa</em> biofilm.</p> <p>It was also observed that tested compounds show high antibiofilm activity in comparison to reference antimicrobials.</p> <p>The aminopropanol derivatives may provide templates for a new group of antimicrobial agents and potential future therapeutics for treating chronic infections.</p&gt;}, number={1}, journal={Farmatsevtychnyi zhurnal}, author={Dudikova, D. M. and Suvorova, Z. S. and Nedashkivska, V. V. and Sharova, A. O. and Dronova, M. L. and Vrynchanu, N. O.}, year={2018}, month={Aug.}, pages={93-100} }