Research of mushrooms lectins of Russulaceae family. A genus Lactarius
Abstract
In search of lectins 13 species of fruiting bodies from Lactarius genus mushrooms have been investigated. The lectins were found in 9 species (L. vellereus, L. pergamenus, L. piperatus, L. rufus, L. torminosus, L. volemus, L. deliciosus, L. necator, L. quietus). Carbohydrate specificity of lectins from 8 Lactarius species were investigated. All investigated lectins were inhibited by group-specific substances of human blood, bovine thyroglobulin and human immunoglobulin G.
High lability is characteristic of lectins from Lactarius species mushrooms. Hemagglutination activity is strongly reduced by drying, freezing, precipitation by ammonium sulphate and ethanol, dialysis against water and buffer solutions. The possible mechanism of lectin lability is being discussed.
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