HPLC procedure validation of azithromycin determination in medicines and comparison of it with spectrophotometric procedure by reaction with 2-nitrobenzaldehyde.
Keywords:
high performance liquid chromatography, spectrophotometry, 2-nitrobenzaldehyde, azithromycin, validation, F-criteria
Abstract
It is introduced and validated a definition procedure of azithromycin by a high performance liquid chromatography. Comparison of results of the analysis of a series a medical product in form of capsules containing azithromycin, received according to introduced HPLC procedure and that preliminary developed by spectrophotometric on reaction with 2-nitrobenzaldegidom by means of F-criterion is spent. Both considered techniques allow to receive results which statistically do not differ.
References
1. USP30-NF25, P.1472.
2. SA Shklyaev, OUR Turkan, OP Kolyadich, OO Kulikova // Pharmaceutical Journal. - 2010. - № 6
- P. 50-55.
3. Validation of analytical methods and tests // State Pharmacopoeia of Ukraine / State Enterprise "Scientific Expert Pharmacopoeial Center". - Type 1. - X .: RIREG, 2001. - P. 58-67. - Supplement 1. - 2004. - P. 2-4. - Supplement 2 - 2008. - P. 85-100.
4. K. Durffel. Statistics in analytical chemistry. - Per. with him. - M .: Mir, 1994.
5. Grizodub AI // Pharmacom. - 2002. - № 3 - P. 42-50.
6. AI Grizodub, DA Leontiev, NV Denisenko, Yu.V. Podpruzhnikov // Pharmacom. - 2004. - № 3
- P. 3-17.
2. SA Shklyaev, OUR Turkan, OP Kolyadich, OO Kulikova // Pharmaceutical Journal. - 2010. - № 6
- P. 50-55.
3. Validation of analytical methods and tests // State Pharmacopoeia of Ukraine / State Enterprise "Scientific Expert Pharmacopoeial Center". - Type 1. - X .: RIREG, 2001. - P. 58-67. - Supplement 1. - 2004. - P. 2-4. - Supplement 2 - 2008. - P. 85-100.
4. K. Durffel. Statistics in analytical chemistry. - Per. with him. - M .: Mir, 1994.
5. Grizodub AI // Pharmacom. - 2002. - № 3 - P. 42-50.
6. AI Grizodub, DA Leontiev, NV Denisenko, Yu.V. Podpruzhnikov // Pharmacom. - 2004. - № 3
- P. 3-17.
Published
2019-09-10
How to Cite
Shkliaiev, S., Tsurkan, A., Koliadich, E., & Kulikova, O. (2019). HPLC procedure validation of azithromycin determination in medicines and comparison of it with spectrophotometric procedure by reaction with 2-nitrobenzaldehyde. Farmatsevtychnyi Zhurnal, (1), 64-68. Retrieved from https://pharmj.org.ua/index.php/journal/article/view/711
Section
Original Articles
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