Influence of «Melanizol» and «Klimedeks» pessaries on phagocytic activity of polymorphonuclear leukocytes in vitro

Keywords: pessaries, vaginitis, phagocytic activity, polymorphonuclear leukocytes

Abstract

Local immunity during aerobic vaginitis is provided by the functioning of the mucous membranes, the secretory immune system and cells that have the ability to phagocytosis. Neutrophils related to polymorphonuclear leukocytes (PNL), involved in phagocytosis reactions, are markers of inflammation and prevail in the vagina of patients with an infection.

The aim of the work was to study the effect of new vaginal pessaries «Melanizol» and «Klimedeks» on the phagocytic activity of PNL in vitro.

The study of the influence of «Melanizol» and «Klimedeks» pessaries on the activity of PNL to phagocytize cells of yeast in vitro has been investigated. Reference drugs were «Gravagin» pessaries and vaginal tablets «Mikozhynax» and «Neotrizol». Determination of phagocytic activity (FA) of neutrophils was investigated according to generally accepted parameters: phagocytic index (FI) and phagocytic number (FN). Statistical processing was performed using the «Statistica 6.0» package using the Mann-Whitney Criterion the Mann-Whitney Criterion.

FI significantly increased under the influence of pessaries «Kimedeks» and tended to increase under the influence of pessaries «Melanizol» (1.24 and 1.18 times, respectively) in comparison with intact samples. A significant increase in the FN under the influence of means «Melanizol» and «Klimedeks» was observed at 33% and 30%, respectively, during the study. «Melazinol» and «Klimedeks» pessaries significantly increased the FA in comparison with «Neotrizol» and «Gravagin» reference drags in vitro. The reference drug «Mikozhynax» did not have an effect on PNL in an experiment on the study of the effect on phagocytic activity in vitro.

According to the results of in vitro studies, the pessaries «Melanizol» and «Klimedeks» influenced on the phagocytic activity of polymorphonuclear leukocytes, increased the phagocytic index and phagocytic number in comparison with intact samples. The studied pessaries were superior to the reference drugs «Gravagin», «Neotrizol» and «Mikozhynax». Thus, the investigated pessaries «Melanisol» and «Klimedeks» can be studied as drugs for the treatment of vaginitis with a reduced immune status.

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Published
2019-07-01
How to Cite
Dolzhykova, O. V., & Maloshtan, L. M. (2019). Influence of «Melanizol» and «Klimedeks» pessaries on phagocytic activity of polymorphonuclear leukocytes in vitro. Farmatsevtychnyi Zhurnal, (3), 96-101. https://doi.org/10.32352/0367-3057.3.19.11
Section
Pharmacology