Spasmolitine kinetic spectrophotometric assay

  • M. Ye. Blazheyevskiy -
  • L. S. Kryskiw -
Keywords: spasmolitine, perhydrolysis, spectrophotometric method, quantitative assay

Abstract

A kinetic spectrophotometric method has been developed for the determination of Spasmolitine using the indicator reaction of catalytic 3,3',5,5'-Tetramethylbenzidine oxidation by hydrogen peroxide at pH 8,5 in pure substance. Calibration graph for Sp has linear dependence in the range 0,01 – 0,15 mg/ml, a limit of quantitation (LOQ) is 3,9∙10-5 mol·dm-3 Spasmolitine. At Spasmolitine concentration 0,40 mmol·l-1  determinations the reproducibility has a relative S. D. ≤3,33% (δ =-0,68%). The proposed method is more sensitive, simple and expresses in comparance with the well-known one. Sp substance contains 99,30±4,05%. of C20H25NO2,HCl.

References

1. Beklemishev M.K. New indicator systems in kinetic methods of analysis. Sorption-kinetic method. Author's abstract. diss ... doc. chem Sciences: special 02.00.02 «Analytical chemistry». - M., 2011. - 43 p.
2. State Pharmacopoeia of Ukraine / State Enterprise "Scientific Expert Pharmacopoeia Center". - The first view. - Kharkiv: RIREG, 2001. - 556 pp.
3. Durffel K. Statistics in Analytical Chemistry. - M .: Mir, 1994. - 267 pp.
4. Kryzhanovsky C. A., Vitytnova M. B. A complete modern handbook of drugs. Practical guide: 2nd ed., Pererab. and add - Moscow: RIPOL CLASSIC, 2002. - 1216 pp.
5. Lazarev A.I., Kharlamov I.P., Yakovlev P. Y. and others. Handbook of chemical analyst. - Moscow: Metallurgy, 1976. - 184 p.
6. Mashkovsky M. D. Medicines: In 2 t. T. 1. - 14 th ed., Pererab., Correction. and add - Moscow: New Volna, 2002. - 540 p.
7. Tikhonova O.I., Yarniy T.G., Gritsenko S.V. and others. Solid dosage forms: Extemporal formulation: Methodical recommendations. - Haoques: View of the NFUU, Golden Pages, 2003. - 176 p.
8. Turkevich MM Pharmaceutical Chemistry. - K .: High school, 1973. - 494 pp.
9. Eksperandova L.P., Belikov K. N., Khimchenko S. B. et al. // THE STORY - 2010. - T. 65, No. 3. - P. 229-234.
10. Cserháti T., Forgács E. // J. Chromatogr. B Biomed. Sci Appl. - 1998. - V. 717, No. 1-2. - P. 157 - 178.
11. Issa Y. M., Ibrahim Hosny, Shoukry A. F. et al. // Microchemical J. - 1990. - V. 42, N 3. - P. 267-273.
12. Laurent Y. Galichet. Clark's Analysis of Drugs and Poisons: Third Edition. - 80 min / 700 MB. - Pharmaceutical Press, 2005. - 1 electron. wholesale CD (CD-ROM); 12 cm - Systems. requirements: Pentium; 128 Mb RAM; CD-ROM; Windows XP / Vista. - Title with title. the screen.
Published
2019-03-18
How to Cite
Blazheyevskiy, M. Y., & Kryskiw, L. S. (2019). Spasmolitine kinetic spectrophotometric assay. Farmatsevtychnyi Zhurnal, (6), 67-73. Retrieved from https://pharmj.org.ua/index.php/journal/article/view/450
Section
Original Articles