Study of hypoglycemic efect of raleykin under experimental diabetes of type I in rats

  • I. P. Bukhtiyarova Donetsk National Medical University of M. Gorky
  • S. M. Drogovoz National University of Pharmacy, Kharkiv
  • О. M. Ishchenko Scientific-Research Institute of Pure Biochemicals, Saint-Petersburg, Russia
Keywords: diabetes, hypoglycemic action, raleykin

Abstract

The aim of this article was the study the lipid-lowering properties of the drug - antagonist receptor interleukin-1 – raleykin. The medication was got by recombinantly way in St. Petersburg Scientific-Research Institute of Pure Biochemicals. This is due to the fact that the pharmaceutical formulations in Ukraine no such class. However, the mechanisms of diabetes mellitus indicate the necessity for such drugs. Investigations were carried out on the model of diabetes mellitus.

The model was reproduced by low-dose streptozotocin, as it causes a pathophysiological signs of type I diabetes in humans (insulin-dependent growth, b-cell necrosis and hyperglycemia).

Simulation result was the increase of blood glucose levels compared with the control – by 111.4%. The area under the curve of glycemia in diabetes group was more than 95.6%. Comparison of the results obtained at the end of the experiment, between the group of animals treated with reference medication – anakinra and diabetic group showed that, after a preparation is less than the level of glucose was 72.7%. The area under the glycemia curve in a group of animals receiving anakinra, was reduced by 15.6%. The experiment had showed that administration of raleykin in therapy regimen experimental rats model of type 1 diabetes is significantly reduced blood glucose levels (79.6%). Meaning of the area under the glycemia curve that determines the tolerance for carbohydrates, decreased as well as the application of the reference preparation – anakinra (15.6%). The comparing effects of both medications revealed it was shown that for these indicators significant difference between the drugs is absent.

Thus, further studies raleykin as an anti-diabetic medication are very relevant, since no one drug – receptor antagonist, interleukin-1 in Ukraine not registered.

References

1. WHO Newsletter. - 2013. - № 312. Access mode - http://www.who.int/mediacentre/factsheets/fs312/en
2. Tikhonova T.M. Comparative evaluation of cytokine content in patients with slowly progressive autoimmune diabetes in adults // Problems of endocrine pathology. - 2013. - № 1. - P. 27-34.
3. Skibchik VA, Gutor Ya.G., Voinovich M.O. and others. Proinflammatory cytokines in diabetes mellitus type 1 // Experimental. the clinic physiol biochemistry - 2012. - No. 1. - P. 57-60.
4. Kravets E. B., Kaprin T. V., Lazarenko F. E. et al. The role of cytokines in the pathogenesis of autoimmune diabetes, questions of immune intervention. // Bull. Siberian medicine. - 2010. - № 1. - P. 76-83.
5. Suprun E. B. Correction of Interleukin-1 Receptor Antagonist Indicators of Energy Metabolism in the Brain of Rats with Experimental Ischemic Stroke // Theor. experiment medicine. - 2010. - No. 1. - P. 64-68.
6. Rodinsky O.G., Guz V.A. Mechanisms of the formation of defeat of the central and peripheral nervous system under conditions of experimental diabetes // Medical perspectives. - 2009. - T. XIV, № 3. - P. 4-14.
7. Preclinical research of drugs: Method. recommendations / edited by O. V. Stefanov. - K .: Avicenna, 2001. - P. 396-409.
8. Schwarznau A., Hanson MS, Sperger J. M. Et al. IL-1β Receptor Blockade Protects Islets Against Pro-Inflammatory Cytokine Induced Necrosis and Apoptosis // J. Cell Physiol. Author manuscript. - 2009. - V. 220, N 2. - P. 341-347.
9. Khokhla M., Kleveta G., Chayka Ya and others. Influence of medicinal gleb on apoptosis of leukocytes of rats under conditions of experimental type 1 diabetes mellitus // Visn. Lviv Univ. Biological series. - 2012. - Bip. 60. - P. 117-125.
10. Ferents IV, Brodyak IV, Luta M. Ya. Et al. Effect of agmatine on blood system parameters of rats under conditions of experimental diabetes // Biological Studios / Studia Biologica. - 2012. - Vol. 6, No. 3. - P. 65-72.
Published
2019-02-12
How to Cite
Bukhtiyarova, I. P., Drogovoz, S. M., & IshchenkoО. M. (2019). Study of hypoglycemic efect of raleykin under experimental diabetes of type I in rats. Farmatsevtychnyi Zhurnal, (5), 99-104. Retrieved from https://pharmj.org.ua/index.php/journal/article/view/381
Section
Pharmacology