Spectrophotometric method of quantitative determination of warfarin clathrate sodium in substance and tablets
Abstract
Warfarin sodium clathrate is widely used as a therapeutic agent for inhibiting blood coagulation (group of indirect anticoagulants), preventing the synthesis of vitamin K-dependent factors. Its effect is cumulative, so toxicity is usually the result of regular use.
We have studied drug used in tablet form to the content of the active substance warfarin sodium clathrates under 2.5 or 3 mg.
The aim of the study was to investigate the possibility of quantitative determination of warfarin sodium clathrates in substance and tablets by ultraviolet spectrophotometry.
We studied the UV spectra of clathrate warfarin sodium tablets and warfarin with a spectrophotometer SPECORD 200-222 U 214.
As the solvent is used: purified water, 95% ethanol, methanol, 0.1 M solution of hydrochloric acid, 1 M solution of hydrochloric acid, 0.1 M solution of sulfuric acid, 1 M solution of sulfuric acid, 0.1 M sodium hydroxide; 1 M solution of sodium hydroxide, acetonitrile, chloroform, cyclohexane. The choice of these solvents is caused by following factors: a) the frequent use of certain solvents (ethanol, methanol, acetonitrile, cyclohexane, chloroform) to highlight the substance of dosage forms and biomaterial b) the necessity of selection of solvents that form a solution with the highest absorption value for use to quantify c) a possibility of formation of salts in solutions of hydrochloric, sulfuric acid and sodium hydroxide, and detection of hydrolytic processes in acidic and alkaline environments.
The UV spectra of warfarin is studied in purified water, 95% ethanol, methanol, 0.1 M solution of hydrochloric acid, 1 M solution of hydrochloric acid, 0.1 M solution of sulfuric acid, 1 M solution of sulfuric acid, 0.1 M sodium hydroxide , 1 M sodium hydroxide, acetonitrile, chloroform, cyclohexane. It is found that the UV spectra of warfarin in these solvents are characterized by two absorption bands. The first band has a maximum in the range of 201 to 241 nm, the second - in the range 305-319 nm.
A method is proposed to quantify substance warfarin sodium clathrates (water at 306 nm, 95% ethanol at 312 nm in acetonitrile at 319 nm) and tablets (95% ethanol at 312 nm, acetonitrile).
The error of determination of warfarin sodium clathrates in the substance does not exceed ± 1.3876, and tablets ± 2.1787, showing the reliability of the results.
References
2. Mashkovsky M. D. Medicines. - M .: The New Wave, 2010. - 1216 p.
3. Prokhorova AF Macrocyclic modifiers for increasing the selectivity of the electrophoretic separation of enantiomers of organic acids. Author's abstract. dis ... Candidate chem sciences Special 02.00.02 - "Org. chemistry ". - M., 2010. - 22 p.
4. Smirnova T.D., Nevryueva NV, Shytykov SM, and others. Determination of warfarin by sensitized fluorescence method with the use of organized media // Zh. analyte ximia - 2009. - T. 64, No. 11. - P. 1114-1119.
5. Tkacheva N.A. Investigation of influence of Vkorc 1c1173T genotype on pharmacokinetic parameters of warfarin metabolism // Rus. honey. journ - 2005. - No. 1. - P. 15-17.
6. Flanagan R. J. Fundamentals of Analytical Toxicology. - Geneva: WWO, 1999. - 363 pp.
7. Fasco M.J., Cashin M.J., Kaminshy L.S./A New Method for Quantitation of Warfarin and its Metabolites in Plasma // J. Liq. Chrom - 1979. - V. 2. - P. 565-575.
8. Guan F., Ishii A., Sen. H. et al. A method for simultaneous determination of five anticoagulant rodenticides in whole blood by high-performance liquid chromatotography // J. Phaem. Biomed Anal - 1999. - V. 21. - P. 179-185.
9. Kollroser M., Schober C. Determination of coumarin-type anticoagulants in human plasma by HPLC-electrospray ionization. Tandem mass spectrometry with an ion trap detector // Clin. Chem - 2002. - V. 48. - P. 84-91.
10. Loomis C. W. Determination of warfarin in plasma by gas-liquid chromatography // Anal. Chem Acta - 1979. - Vol. 106. - P. 155-159.
11. Maurer H. H. Detection of 4-hydroxycoumarin anticoagulants and their metabolites in urine as part of a systematic toxicological analysis procedure for acidic drugs and poison gas chromatography spectrometry after extractive methylation // J. Chrom. B. - 1998. - V. 714. - P. 181-195.
12. Midha F. K. K., Gilver I. J. Mc., Coope J. K. GLC Determination of Plasma Levels of Warfarin // J. Pharm. Sci - 1974. - V. 63. - P. 1725-1729.
13. Odam E. M., Townsend M. G. The determination of warfarin in animal tissues by electron capture gas liquid chromatography // Analyst. - 1976. - V. 101. - P. 478-484.
14. Osman A., Arbiing K., Lindahl T. L. A new high-performance liquid chromatography method for the determination of warfarin enantiomers // J. Chrom. B. - 2005. - Vol. 826. - P. 75-80.
15. Tasrer R. A. R., Nakatsn K. Rapid, A reliable and sensitive assay for warfarin using normal-phase high-performance liquid chromatography // J. Chrom. - 1982. - V. 228. - P. 346-349.
16. Ufer M., Kammerer B., Kirchheiner I. et al. Determination of phenprocomon, warfarin and their monohydroxylated metabolites in human plasma and urine by liquid chromatography-mass spectrometry after solid-phase extraction // J. Chrom. B. - 2004. - V. 809. - P. 217-226.
17. Uno T., Niioka T., Hayakari M. et al. Simultaneous determination of warfarin enantiomers and its metabolites in human plasma by column-switching high-performance liquid chromatography with chiral separation // Ther. Drug Mon - 2007. - P. 29. - P. 333-339.
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