Studies of heparine solutions preserved in glass and plastic ampoules by the metod of laser desorption mass spectrometry

  • O. V. Severinovskaya Chuiko Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Kуіv
  • N. I. Karandeyeva Pharmaceutical company «НИКО», Маkееvkа, Donetsk region
  • I. N. Osipova Pharmaceutical company «НИКО», Маkееvkа, Donetsk region
  • A. G. Lisitskiy Pharmaceutical company «НИКО», Маkееvkа, Donetsk region
Keywords: heparin, MALDI ToF mass spectrometry, desorption


In this study the practical problem of permissibility of heparin preservation in ampoules made of various materials was investigated by methods of matrix-assisted and matrix-free laser desorption/ionization combined with time-of flight analyzer. 

It was shown that in the case of investigation of fabricated solutions of heparin by MALDI ToF method the investigation of heparin is restricted by presence of protein-stabilizer. Thus, the method of choice for investigation of heparin preservation in ampoules is the matrix-free laser desorption mass spectrometry.

It was found out that heparin degradation takes place during the period of preservation in opened ampoules which fact is confirmed by increase of fragments number of structural units of heparin and this process is characteristic both for glass and plastic ampoules during the whole period of measurements.

It has been also shown that, according to results of mass spectrometric analysis (in particular to character of obtained mass spectra and number of desorption products) the evolution of heparin from the surface of glass and plastic ampoules was almost similar during 14 days. Thus, plastic may be used as alternative to glass as material for fabrication of ampoules.


1. Gobom J., Nordhoff E. High-throughput proteomics using matrix-assisted laser desorption / ionization mass spectrometry // Expert Rev Proteomics. - 2005 -V 2, Issue 3 - P. 407-420.
2. Hanjo L., Eng J., Yates III J. R. et al. Identification of 2D-gel proteins: A comparison of MALDI / TOF peptide mass mapping to μ LC-ESI tandem mass spectrometry // J. Amer. Soc Mass Spectrom. - 2003. -V. 14, Issue 9 - P. 957-970.
3. Decina S. S., Romanovskaya I. I., Gromova T. Yu. The influence of polymers on the processes of association of lysozyme molecules // Biopolymers and Cell. - 2011. - V. 27, N 6. - P. 442-445.
4. Andrusishina I. N., Golub, I. A., Didikin G. G., et al. Structure, properties and toxicity of nanoparticles of silver and copper oxides // Biotechnology. - 2011. - Vol. 4, No. 6. - P. 51-59.
5. Alekseev S.A., Severinovskaya O.V., Gurskaya SV, et al. Modified porous silicon for identification of bile components by the DIOS method // Supplement. National Academy of Sciences of Ukraine. - 2010. - No. 6. - P. 164-168.
6. Severinovskaya O.V., Warzatsky O.A., Shulga SV, et al. Application of ion-forming additive for mass spectrometric analysis and identification of biological objects // Chemistry, physics and surface technology. - 2011. - T. 2, No. 3. - P. 348-351.
7. Snegir SV, Severinovskaya OV, Vlasova N. N. et al. Investigation of the composition of sodium taurocholate associates by mass spectrometry with matrix-activated desorption / ionization // Mass spectrometric journal. - 2007. - No. 4 (2). - P. 99-102.
8. Shmygol IV, Severinovskaya O.V., Vasilieva N.S. et al. Mass spectrometric study of adsorption of histamine and arginine on different surfaces of porous silicon by laser desorption ionization // Chemistry, physics and surface technology. - 2007. - No. 13. - P. 341-349.
9. Bultel L., Landoni M., Grand E. et al. UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography // J. Amer. Soc Mass Spectrom. - 2010. - V. 21, Issue 1 - P. 178-190.
10. Ket. W. S., Coombe D. R. A structural analysis of heparin-like glucoseaminoglycans using MALDI-TOF mass spectrometry // Spectroscopy. - 2004 - V. 18, Issue 2 - P. 185-201.
11. Przybylski C., Gonnet F., Bonnaffé D. et al. HABA-based ionic liquid matrices for UV-MALDI-MS analysis of heparin and heparan sulfate oligosaccharides // Glycobiology. - 2010. - Vol. 20, Issue 2. - P. 224-234.
12. European Pharmacopeia. 7th ed. Monographs HEPARINS, LOW-MOLECULAR-MASS. - 2011 – 2151 p.
How to Cite
Severinovskaya, O. V., Karandeyeva, N. I., Osipova, I. N., & Lisitskiy, A. G. (2019). Studies of heparine solutions preserved in glass and plastic ampoules by the metod of laser desorption mass spectrometry. Farmatsevtychnyi Zhurnal, (4), 76-82. Retrieved from
Production, quality control, standardization of medicines