Study of qualitative and quantitative composition of lipophilic compounds in the extract of pumpkin pulp (Cucurbita pepo L.)

  • L. I. Vyshnevskaya National University of Pharmacy, Kharkiv
  • E. A. Degtyaryovа National University of Pharmacy, Kharkiv
  • Y. I. Bysaha Uzhhorodskyе National University
Keywords: waste product, herbal raw material, pumpkin, pulp, chemical composition, lipophilic substances


The extracts of medicinal plants included into the composition of many drugs as active substances. Due to the presence in plants of different classes of biologically active substances with different pharmacological effects, it is possible to usethem for the treatment and prevention of many diseases. One of such plantsis pumpkin (Cucurbita pepo L.), which belongs to the family Cucurbitaceae.

The aim of our work was investigation and studying at the chemical composition of biologically active compounds of the lipophilic extracts of pumpkin by the method of chromatography-mass spectrometry.

The object of the study was lipophilic extract of pumpkin pulp meal which had been prepared by using the hexane as solvent (1:6).

The cake of pumpkin pulp from the (Cucurbita pepo L. and Cucurbita moschata (Duch) Poir.) after juice production in 2013 was the raw material for producing at the extract.

In the lipophilic extract of pumpkin pulp 37 compounds. Of the seare present 10 fattyacids, of which 7 – saturated (lauric, caproic, myristic, palmitic, pentadecanoic, heptadecanoic, behenic), 2 – polyunsaturated (linoleicand, linolenic), 1 – monounsaturated (oleic). The greatest number of identified palmitic (6 232.6 mg/kg) and oleic (4 158 mg/kg) acids. It was also β-tocopherol (vitamin E) in an amount of 1 102.3 mg/kg and squalene (210.5 mg/kg) were identified.

The development of drugs for the prevention and treatment of various diseases is perspective due to the presence and big number of identified compounds in the lipophilic extract of pumpkin pulp.


1. Ostrovskaya A. M. Development of the production of phytopreparations in the Republic of Belarus on the basis of domestic vegetable raw materials // Proceedings of the Research Institute of Plant Protection of the BSMU. - 2006. - pp. 856-859.
2. Zheleznov AV Amaranth - bread, spectacle and medicine // Chemistry and life. - 2005. - No. 6. - P. 56-61.
3. Biisko N. A., Barshtein V. Yu., Krupodirova T. A., Ivanova T. S. Amino acid composition of bioconventional products of amaranth seed meal with higher mushrooms // Problems of nutrition. - 2009. - No. 3-4. - P. 53-58.
4. Formazyuk V. I. Encyclopedia of food medicinal herbs: cultural and wildlife plants in practical medicine / Ed. N. P. Maksyutina. - K .: Izdv-o A.K., 2003. - 792 p.
5. Gavris, I. L. Biochemical indices of tomato fruit for use of plant growth regulators // Scientific supplement. NAU - 2007. - No. 1 (6). - P. 10-12.
6. Husid S. B., Nesterenko E. E., Zholobova I. S. Changes in the chemical composition of pumpkin fruits during storage // SWorld. - 2012. - pp. 2-12.
7. Hata K. et al. Effects of Pumpkin seed extract on urinary bladder function in anesthetized rats // Med. Science and Pharm. Science - 2005. - N 54 (3). - R. 2-10.
8. Hana H. Ahmed, Mona A. R. Protective effect of Ginkgo Biloba extract and Pumpkin seed oil on neurotoxicity of rotenone in adult male rats // J. Appl. Sci Res - 2009. - N 5 (6). - P. 622-635.
9. Harmonov S. Yu., Shitova N. S., Yusupova L. M. Quality control and safety of medicines: sch. Handbook / Ed. S. Yu. Harmonova. - Kazan: Izan Kazan. state techno Un-ta, 2008. - 171 pp.
10. Krylov VA, Chernova O. Yu., Sozin A. Yu, and others. Chromat-mass spectrometric determination of impurities in high purity phosphine using capillary adsorption chromatographic columns // Analytics and control. - 2013. - Vol. 17, No. 4. - P. 452-458.
11. Polunin K. E., Voytova V. M., Buryak A. K., and others. Chromat-mass spectrometric study of thermosorbtion of immobilized biologically active compounds. Sorption and chromatographic processes. - 2010. - T. 10. Extract 25. - p. 273-282.
12. Mamedova S.O., Pavlyi O.I. Study of lipophilic compounds of vegetative organs of strawberry forest // Med. chemistry. - 2011. - No. 3 (48), T. 13. - P. 44-46.
13. Deineka L. A., Deineka V. I., Goshchev, I. A., and others. Definition of squalene in seeds of some plants of the family Amaranthaceae // Chemistry rasst. raw materials - 2008. - No. 4. - P. 69-74.
14. Cheukunova Yu.A., Zykovich S.N. Efficiency of use of feed additives from sorrel of Rumek k-1 grade and Amantarum "Yantar" for increasing productivity of sows and piglets // West. Altai State agrarian un-ta. - 2012. - No. 9 (95). - P. 86-88.
15. Dzyuba VF, Safonova E. F., Frolova IV. Biopharmaceutical research of medicinal forms with amaranth oil // West. VSU, Chemistry Series. Biology. Pharmacy - 2007. - No. 2. - P. 145-150.
16. Krasnokutsky SV, Shaporenko SV Vitamin E in the prophylaxis and treatment of conditions associated with the activation of peroxide oxidation of lipids. / Original research. - 2010. - No. 1-2. - P. 109-113.
How to Cite
Vyshnevskaya, L. I., DegtyaryovаE. A., & Bysaha, Y. I. (2019). Study of qualitative and quantitative composition of lipophilic compounds in the extract of pumpkin pulp (Cucurbita pepo L.). Farmatsevtychnyi Zhurnal, (4), 47-52. Retrieved from
Pharmacological, phytochemical research