Investigate of extractions and chromatographyc conditions gydazepam’s aminobenzophenones

  • M. A. Savchenko The Cherkassy regional bureau of a forensic medicine
  • G. P. Petyunin Kharkiv Medical Academy of Post-graduate Education
Keywords: gydazepam, aminobenzophenones, extraction, thin layer chromatography


Detection aminobenzophenones, after hydrolysis of a biological tissue, represents one of stages of screening both the derivatives of 1,4-benzodiazepine, and the research on «general unknown». One of factors of successful detection and identification is the knowledge of physical and chemical properties of required substances.

The purpose of this work, to study influence of the nature of extragent and enviroment acidity on extraction gydazepam’s aminobenzophenones from hydrolysates and also to find out their chromatographic behaviour in the conditions of thin-layer chromatography.

Model solutions gydazepam’s aminobenzophenones in buffer solutions and liver hydrolysate were objects of research. A quantitative assessment made a gas-liquid chromatography method. The chromatographic behaviour was studied in twenty systems for thin-layer chromatography.

In findings of investigation it is discovered that extraction of the most typical aminobenzophenone of gydazepam, considerably influences рН, whereas extraction of other two gydazepam’s aminobenzophenones, рН practically does not influence. Studying of the nature of extragent has shown possibility of application of all most widespread solvents in laboratory practice. The exception is only by non-polar hexane and heptane. Chromatographic fractionation gydazepam’s aminobenzophenones in various systems has shown significant distinctions between them in chromatographic mobility. Also discovered that on their identification influence aminobenzophenones from other derivatives 1,4-benzodiazepinе.

It is discovered that optimal conditions of extraction gydazepam’s aminobenzophenones are рН 5, extragent n-chlorbutan (and also dichloromethane and chloroform). In the conditions of the classical schema of screening derivatives 1.4-benzodiazepine, difficulties in detection and identification gydazepam’s aminobenzophenones can arise a thin-layer chromatography method.


1. Zhuk O. V., Karpinchik V. A. Gidazepam - a new domestic daytime tranquilizer // Provizor. - 2000. - No. 17. - P. 33-35.
2. Andronati S.A., Voronina T.A., Golovenko N.Ya. and others. Guidazepam. - K .: Science. Opinion, 1992. - P. 15-16, 21, 50-52, 70-75, 63-75, 120-131.
3. Savchenko MA, Petyunin GP Research on the behavior of hydazepam and its metabolites in acid hydrolysis // Bukovinsky honey. Visn - 2013. - T. XVII, No. 3 (67), Ch.1 - P. 143-148.
4. Golovenko N.Ya., Zinkovsky V.G., Andronati S.A. Mass spectrometric analysis of derivatives of 2-aminobenzophenones and their metabolites // Bioorgan. chemistry - 1986. - No. 6. - P. 88-91.
5. Bartov A.I., Voronova NV, Koshelev A.S. Isolation and determination of various narcotic and medicinal substances after acid hydrolysis of biological material (report 1) // Forensic medical examination. - 1998. - No. 6. - P. 26-27.
6. Eremin S. K., Isotov B. N., Veselovskaya N. V. Analysis of narcotic means. - Moscow, 1993. - P. 47, 188-194.
7. Preston H. "Capillary flow technology for GC / MS: a simple path analysis for trace concentrations with rapid backflushing for matrix elimination". Agilent Technologies publication 5989-8664EN.
8. Recommended Methods for Detection and Assay of Benzodiazepines in Biological Samples Manual for Use by National Laboratories: ST / NAR / 27. - N.Y .: United Nations, 1997. P. 78.
9. Schtitz H. Benzodiazepines II - a handbook. - Berlin, Heidelberg, New York, London, Paris, Tokyo: Springer, 1988. - P. 47-48.
10. Thin-Layer Chromatographic Rf Values of Toxicologically Relevant Substances in Standardized Sysmtes; 2nd Ed. Deutsche Forschungsgemeinschaft (DFG), International Association of Forensic Toxicologists (TIAFT) Copyright 1992 VCH Verlagsgesellschaft mbH, Weinheim. - P. 306-308.
11. Clarke's Analysis of Drugs and Poisons in pharmaceuticals, body fluids and postmortem material fourth edition. - P. 612-615.
How to Cite
Savchenko, M. A., & Petyunin, G. P. (2019). Investigate of extractions and chromatographyc conditions gydazepam’s aminobenzophenones. Farmatsevtychnyi Zhurnal, (4), 40-46. Retrieved from
Synthesis and analysis of biologically active compounds