Determination of amino acids content in the flowers of the sorts of chrysanthemum (Chrysanthemum xhortorum Baileyl.)
Abstract
Amino acids play important roles in the human body. They are the building units of proteins.
The aim of our researches was to study amino acids content of the flowers of Chrysanthemum sorts as Finos, Grandeur, Apro, Belgo, Ostora. Plants were grown on the experimental plots of Botanical Garden of SHEI «Ternopil I.Ya. Horbachevsky State Medical University of the Ministry of Public Health of Ukraine». Investigation of amino acids content were performed with HPLC method on the Agilent Technologies 1100 chromatograph.
As a result of HPLC analysis it was established 19 amino acids: 8 (threonine, valine, methionine, isoleucine, leucine, phenylalanine, histidine, lysine) from them are essential (they income to the body with food), 11 (glutamine, asparagine, serine, arginine, glycine, alanine, proline, cysteine, tyrosine, asparagine, glutamic acid) are nonessential amino acids in the flowers of Chrysantemum sorts as Finos, Grandeur, Apro, Belgo, Ostora.
Among the essential amino acids the most abundant components were threonine – 355,3 mg/100 g in the flowers of Chrysantemum of Finos sort, 340 mg/100 g in Apro sort and 320,1 mg/100 g in Belgo sort.
Also in the high quantity it were established the bounded form of leucine – 351,0 mg/100 g, lysine – 344,2 mg/100 g and phenylalanine – 311,5 mg/100 g in the flowers of Chrysantemum of Finos sort.
The flowers of all Chrysantemum sorts contain nonessential amino acid – proline. So the content of this amino acid were 2 475,6 mg/100 g in the flowers of Chrysantemum of Finos sort, 2 626,3 mg/100 g in Grandeur sort, 2 939,0 mg/100 g Belgo sort, 2 356,1 mg/100 g in Ostora sort.
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