Identification and quality control glucosamine hydrochloride in gel for treatment of wounds in II the phase of process from wounds
In order to develop methods of the quality control of a gel for the treatment of wounds in the II phase of process from wounds the research concerning identification of biologically active substance – glucosamine hydrochloride.
Objects of research were laboratory and laboratory-industrial designs gel with allantoin, glucosamine hydrochloride and essential oil of lavender. For determination of mass fraction of glucosamine hydrochloride used a method of high performance liquid chromatography with spectrophotometric detector. This method allows both the identification and quantification. The developed technique of quantitative definition is based at the Department of glucosamine hydrochloride from the main mass of the components of the gel by the distribution of samples in the moving phase – phosphate buffer solvent–acetonitrile with subsequent determination of mass fraction of substances.
When identifying glucosamine hydrochloride on the chromatogram of the investigated solution retention time of the main peak must comply with the time of peak hold glucosamine hydrochloride on the chromatogram of the reference solution. The quantitative determination of the content of glucosamine hydrochloride in 1 of the gel should be in the range from 9.5 to 10.5 mg.
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