Determination of caffeine with the use of effects of the luminescence Tb (III) ion quenching

  • S. V. Beltyukova Odesa National Academy of Food Technologies
  • О. I. Teslyuk Bogatsky Physico-Chemical Institute of National Academi of Sciense of Ukraine, Odesa
  • Е. О. Liventsova Odesa National Academy of Food Technologies
Keywords: caffeine, sorption, sensitized luminescence, terbium (III)

Abstract

Caffeine is a part of many drugs and is widely used in therapeutic practice; therefore, there is the need for careful control of its dosage. For determination of caffeine used spectrophotometric methods, which are not selective, high performance liquid chromatography methods, which require expensive equipment.

The aim of this work was to develop a simple and sensitive methods of quantitative determination of caffeine in pharmaceutical drugs Kofetamin and Piramein quenching effect using caffeine sensitized luminescence of Tb(III) complex with a sorbate 1,10-phenanthroline and β-cyclodextrin in the solid sorbent.

The optimum conditions for obtaining the analytical signal of the proposed luminescent sensor have been investigated. It has been shown that the use of β-cyclodextrin as a second ligand promotes the formation of inclusion complexes of "guest-host", which leads to the displacement of water molecules of the inner sphere of the complex, and to reduce non-radioactive energy loss stimulation. As a result, this significantly increases luminescence. It was established that luminescence sorbate concentration dependent on ion Tb (III) in solution at the maximum concentration 1·10-3 mol/l. Under optimal conditions, the luminescence signal is selected as an analytical band luminescence of Tb(III), corresponding to supersensitive transition 5D47F5emission = 545 nm, λexcitation = 365 nm).

It was shown that most effect of luminescence quenching ion Tb(III) in complexes appears in the sorbent phase - silica gel. The optimal conditions of sorption - sorption time, temperature and drying time was studied.

The observed effect of luminescence quenching of Tb (III) ion in complex with 1,10-phenanthroline and β-cyclodextrin in the presence of caffeine was used by us at the development of methods for determining the of caffeine in dosage formed.

 The content of caffeine was found from calibration carve. The detection limit of caffeine is 0.02 µg/ml. The accuracy and reliability of the determination were verified by statistical processing of the results of determination. The relative standard deviation was 1.7–2.6%.

References

1. Glushchenko N. N., Pleshnev T. V., Ponkov VA Pharmaceutical Chemistry. - Moscow: Academy, 2004. - 320 p.
2. GOST R 51881-2002. Coffee is naturally soluble. General specifications. Introduction 01/01/2003 - M .: Publishing of Standards, 2003. - 12 p.
3. Andreeva K. Yu., Dmitrenko S. G., Zolotov Yu. A. Spectrophotometric determination of caffeine and theophylline by reaction of azo combination with 4-nitrophenyldiasonium tetrafluoroborate // Proceedings. lab Diagnostics of materials. - 2010. - Vol. 76, No. 2. - P. 21-24.
4. Pieszko G., Baranovska I., Flores A. Determination of energizers in energy drinks // Journal. analyte chem - 2010. - T. 12, No. 65. - P. 1257-1263.
5. Kartzova L. A., Alekseeva A. V. The use of selective complexation of catechins with Fe3 + ions in determining caffeine in tea by the method of high-performance thin-layer chromatography // Ibid. - 2009. - T. 64, No. 9. - P. 954-958.
6. Khasanov VV, Dychko KA, Kuryaeva T. T. et al. A new method for determining caffeine // Journal. specimen chem - 2005. - Vol. 78, No. 9. - P. 1451-1454.
7. Golubetskii T. B., Budko E. V., Basov E. M., etc. Chromatographic separation of paracetamol, caffeine and aspirin on sorbent with grafted nitrile groups and analysis of "AscofenP" tablets // Journal. analyte chem - 2007. - Vol. 62, No. 6. - P. 636-640.
8. Papieva I. S., Kirsanov D. O., Legin AV, et al. Analysis of tea samples using the multisensory system and capillary electrophoresis // Ibid. - 2011. - Vol. 84, No. 6. - P. 940-947.
9. Kartsova L. A., Ganzha OV, Alekseeva AV Opportunities and constraints of various modes of capillary electrophoresis for quantitative determination of catechins and caffeine in black and green teas // Ibid. - 2010. - T. 65, No. 2. - P. 212-217.
10. Pylypenko V.P., Bibik O.V., Parish N.N. Comparative characteristic of methods for determining caffeine // Problems of chemistry and chemistry. techno - 2009. - No. 2. - P. 89-91.
11. Egorova AV, Skrypinets Yu. V. Application of sensitized luminescence of lanthanide ions in bioanalysis. - Odessa: Astprint, 2008. - 198 p.
12. Leonenko I.I., Alexandrova D.I., Egorova AV, Antonovich V.P. Analytical application of luminescence stew effects (Review) // Methods and objects of chemistry. analysis - 2012 - Vol. 7, No. 3. - P. 108-125.
13. Kallistratos G., Kallistratos U., Mündner H. Fluorescent properties of aromatic complexes with Rare earts and other elements of (III) a-group // Chim. Chronica New Series. - 1982. - V. 11. - P. 249-266.
14. Khomenko V. S. Investigation of adduct formation of lutetium ion with β-cyclodextrin, diaza-18-crown-6 and 1,10-phenanthroline in solution // Coordination chem. - 1992. - Vol. 18, No. 2. - P. 218-220.
Published
2019-02-12
How to Cite
Beltyukova, S. V., TeslyukО. I., & LiventsovaЕ. О. (2019). Determination of caffeine with the use of effects of the luminescence Tb (III) ion quenching. Farmatsevtychnyi Zhurnal, (2), 84-90. Retrieved from https://pharmj.org.ua/index.php/journal/article/view/306
Section
Original Articles