Lipid peroxidation of rats’ heart with hypertension after nebivolol applying
A significant contribution to the study of the complex mechanisms of the action of beta-blockers may provide the investigation of structure of the heart – the main target organ of hypertension. Recent works have shown that beta-blockers such as carvedilol and bisoprolol, by the deacreasing of blood pressure, reduce adverse morphological changes in the myocardium of rats with spontaneous hypertension (ISIAH). Differences in the effects of various beta-blockers, which are marked in clinical trials are associated with their varying selectivity, sympathomimetic activity, solubility in fats, ability to metabolize in the liver, the duration of action.
By lipid peroxidation was investigated in 3 groups of animals (7 in each group): control (normotensive rats ), rats with ISIAH, ISIAH with rats treated with nebivolol dissolved in water in a dose of 1 mg/kg through gastral tube once a day during 60 days. Blood pressure was measured in the tail artery with a rubber cuff and plethysmograph without anesthesia at the beginning of the experiment and before the withdrawal of animals from the experiment.
Activity lipid peroxidation investigated in mixed arterial-venous plasma and myocardial homogenates. For this registration spontaneous and Fe2+-induced superweak their glow (chemiluminescence) using hemilyuminometr.
Nebivolol statistically significantly had lowered blood pressure in rats with ISIAH till 137 ± 5.0 mm, compared with untreated animals 157 ± 5.0 mm, but did not reach control values 104 ± 1.8 mm. Pressure decrease was correlated with improvement of lipid peroxidation of rat hearts with ISIAH after application of nebivolol. The established difference in the activity of the lipid peroxidation in intact rats and animals hypertensive. In rats with hypertension rats activity of lipid peroxidation in the myocardium is higher compared with normotensive rats, whereas plasma reduced. Nebivolol in the application in rats ISIAH in the myocardium reduces the amount of peroxidation products of free radical reactions in comparison with the control group ISIAH lines. As a result of reduction of reactive oxygen species by reacting with free radicals. Nebivolol in the application within 60 days in rats ISIAH reduces the number of pro-oxidant in plasma.
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