Integrated use of the jerusalem artichoke (Helianthus tuberosus L.) tubers: purification of inulin, fructose and mannosespecific lectin

  • V. O. Antonyuk Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv; Danylo Halytsky Lviv National Medical University
Keywords: inuline, fructose, mannose specific lectin, purification, properties

Abstract

Comprehensive utilization ofraw materialsisan urgent task, allowing their more effectiveuseand helpingto conserve naturalresources.

The aim of this study was to develop a technique co-production of inulin,  fructose and mannose specific lectin from tubers of Jerusalem artichoke.

Ion exchange and affinity chromatography methods, analytical techniques (colorimetry and paper chromatography, polyacrylamide gel electrophoresis), biochemical methods (study the carbohydrate specificity of the lectin) have been used in this work.

It was found that one month after the finishing of the growing season inulin content was less than 3 % from the weight of extractive substances, while the content of fructose and its oligosaccharides was ⅔ of mass of extractives substances. Interaction of mannose-specific lectin with carbohydrates and in particular, with inulin and fructose-containing oligosaccharides was investigated. The author suggests the possible role of the lectin in the transport and accumulation of carbohydrates in the Jerusalem artichoke tubers.

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Published
2019-02-12
How to Cite
Antonyuk, V. O. (2019). Integrated use of the jerusalem artichoke (Helianthus tuberosus L.) tubers: purification of inulin, fructose and mannosespecific lectin. Farmatsevtychnyi Zhurnal, (3), 50-60. Retrieved from https://pharmj.org.ua/index.php/journal/article/view/288
Section
Pharmacological, phytochemical research