Development of approaches to standardization of Helichrysum arenarium (L.) Moench. in multicomponent plant composition

  • A. V. Gudzenko State Institution «Institute of Pharmacology and Toxicology of National Medical Academy of Sciense of Ukraine», Kyiv
Keywords: Helichrysum arenarium (L.) Moench. flowers, standardization, apigenin, multicomponent plant composition, HPLC


Determined marker for qualitative and quantitative standardization of Helichrysum arenarium (L.) Moench. flowers in the plant mixtures. As a marker of Helichrysum arenarium (L.) Moench. flowers can be used apigenin. Apigenin has a wide range of biological activity (in particular, apigenin has anti-inflammatory, antioxidant, anti-cancer and many other pharmacological properties). The method for the analysis of apigenin in Helichrysum arenarium (L.) Moench. flowers and its herbal mixtures using HPLC was developed.

Chromatographic study investigated extracts and reference solution of apigenin was carried out by chromatograph Shimadzu ser. 20, equipped with a diode array detector under the following conditions: column Phenomenex Luna C18 (2) (250 mm × 4.6 mm, 5 micron particle size), the column temperature – 35 °C, detection wavelength – 330 nm, the flow rate of the mobile phase – 1ml/min, volume injection – 5mcl. As the mobile phase used a 0.1% solutions of trifluoroacetic acid in a mixture of water and acetonitrile (gradient elution).

With the developed methodology analyzed of 5 series Helichrysum arenarium (L.) Moench. The analysis shows, that the content of apigenin in raw plant is between 0,1453 ± 0,0068% to 0,1657 ± 0,0085% in terms of the dried materials. It is shown, that the presence and content of apigenin Helichrysum arenarium (L.) Moench. flowers can be standardized in the mixtures with the following plant: leaves and fruits of genus Crataegus L., roots of Althaea officinalis L., roots of Glycyrrhiza glabra L., roots of Cichorium intybus L., roots of Taraxacum officinale Webb., cones of Humulus lupulus L., grass of Leonurus cardiaca L., grass of Hypericum perforatum L. and seeds of Linum usitatissimum L.


1. Dovidnik likarskih zasobiv, zareyestrovanih v Ukrayini stanom na 01. 01. 2014 [Elektronnij resurs]. – Rezhim dostupu:
2. Spravochnik «Kompendium-2011 – lekarstvennye preparaty» / Pod red. V. N. Kovalenko, A. P. Viktorova. – K.: Morion, 2011. – 2270 s.
3. Likarski roslini: Enciklopedichnij dovidnik / Vidp. red. Grodzinskij A. M. – K.: Golovna red. URE, 1989. – 544s.
4. Rastitelnye resursy SSSR: Cvetkovye rasteniya, ih himicheskij sostav, ispolzovanie. Semejstvo Asteraceae (Compositae) / Otv. red. Sokolov P. D. – SPb.: Nauka, 1993. – 351 s.
5. Radusiene J., Janulis V. Improvement of diversity, trade and conservation of medicinal and aromatic plants // Medicina. – 2004. – V. 40, Iss. 8. – P. 705–709.
6. Sroka Z., Kuta I., Cisowski W., Drys A. Antiradical activity of hydrolyzed and non-hydrolyzed extracts from Helichrysi inflorescentia and its phenolic contents // Z. Naturforsch. – 2004. – V. 59, Iss. 5–6. – P. 363–367.
7. Clere N., Faure S., C.Martinez M. Anticancer properties of flavonoids: role in various stages of carcinogenesis // Cardiovasc. Hematol. Agents Med. Chem. – 2011. – V. 9, Iss. 2. – P. 62–77.
8. Cushnie T., Lamb A. Antimicrobial activity of flavonoids // Int. J. Antimicrob. Agents. – 2005. – V. 26, Iss. 5. – P. 343–356.
9. Sandhar H., Kumar B., Prasher S. et al. A review of phytochemistry and pharmacology of flavonoids // Int. Pharm. Sci. – 2011. – V. 1. – P. 25–41.
10. Shukla S., Gupta S. Apigenin: a promising molecule for cancer prevention. // Pharm. Res. – 2010. – V. 27, Iss. 6. – P. 962–978.
11. Gudzenko A. V. Razrabotka metodiki standartizacii kory duba v rastitelnyh smesyah po soderzhaniyu ellagovoj kisloty s ispolzovaniem metoda VEZhH // Voprosy biol., med. farm. himii. – 2013. – № 10. – S 9–12.
12. Lapach S. N., Chubenko A. V., Babich P. N. Statisticheskie metody v mediko-biologicheskih issledovaniyah s ispolzovaniem Excel. – K.: Morion, 2000. – 320 s.
How to Cite
Gudzenko, A. V. (2019). Development of approaches to standardization of Helichrysum arenarium (L.) Moench. in multicomponent plant composition. Farmatsevtychnyi Zhurnal, (6), 77-81. Retrieved from
Pharmacological, phytochemical research

Most read articles by the same author(s)