HPLC-determination of active components in dietary supplement «L-CARNITINE smart»

Keywords: validation, L-carnitine L-tartrate, ascorbic acid, HPLC, dietary supplement

Abstract

Dietary supplements are compositions of biologically active substances intended for consumption with food or addition to food products for the purpose of optimization of metabolic processes and functions of the human body. The dietary supplements include: vitamins, trace elements, amino acids, enzymes, proteins, probiotics, oils that can provide antioxidant, detoxifying, immunomodulatory, adaptogenic effects, etc.

Detection of physiologically active components in dietary supplements is a difficult task and requires the use of modern highly informative research methods. One of the most powerful and versatile methods of determination is the method of high performance liquid chromatography (HPLC), combining the selective separation of the studied mixtures and high sensitivity.

The purpose of this study was to develop a simple, rapid and selective method for determining ascorbic acid and L-carnitine L-tartrate in a multicomponent dietary supplement, produced in the form of sachets, using HPLC with spectrophotometric detection.

The object of the study is the dietary supplement «L-CARNITINE smart», powder for oral solution of 16 g each in a sachet (INTERСHЕM). For the quantitative determination of the applied method of high-performance liquid chromatography. Chromatography was performed on an Agilent 1260 Infinity 2D LC System (USA) liquid chromatograph with a UV detector.

The optimal conditions for the analysis have been experimentally determined: the type of sorbent, the composition of the eluent and its gradient, the wavelength and the detection time for ensuring the release of all components (including auxiliary substances) from the column, selective separation of ascorbic acid, potassium acesulfame and L-carnitine L-tartrate, minimization analysis time. The method has been validated according to the following parameters: specificity, linearity, accuracy, limit of quantitation.

References

1. Slobodskaya N. S. Biologicheski aktivnyie dobavki: znachenie i primenenie // Zhurnal GrGMU. – 2015. – № 4. – S. 119–122.
2. Poznyakovskiy V. M., Chugunova O. V., Tamova M. Yu. Pischevyie ingredientyi i biologicheski aktivnyie dobavki. Uchebnik. – OOO «Nauchno-izdatelskiy tsentr INFRA-M», 2017. – 143 s.
3. Derzhavna Farmakopeya UkraYini / DР «Naukovo-ekspertniy farmakopeyniy tsentr». 1-e vid., dop. 2. – Harkiv: DР «Naukovo-ekspertniy farmakopeyniy tsentr», 2008. – 620 s.
4. Parbhunath O. L., Rautenbach F., Davison G., Marnewick J. L. Optimization and Validation of a Reverse-Phase High Performance Liquid Chromatography Assay with Ultra-Violet Detection for Measuring Total L-Ascorbic Acid in Food and Beverage Products // J. Anal. Bioanal. Tech. – 2014. – V. 5. – P. 201–206. https://doi.org/10.4172/2155-9872.1000201.
5. Mitic S. S., Kostic D. A., Naskovicokic D. C., Mitic M. N. Rapid and Reliable HPLC Method for the Determination of Vitamin C in Pharmaceutical Samples Tropical // J. Pharm. Res. – 2011. – V. 10 (1). – P. 105–111.
6. He G. X., Dahl T. Improved high-performance liquid chromatographic method for analysis of L-carnitine in pharmaceutical formulations // J. Pharm. Biomed. Anal. – 2000. – V. 23. – P. 315–321.
7. McEntyre C. J., Lever M., Storer M. K. A high performance liquid chromatographic method for the measurement of total carnitine in human plasma and urine // Clin. Chim. Acta. – 2004. – V. 344. – P. 123–130. https://doi.org/10.1016/j.cccn.2004.02.010.
8. Qadir M. A., Ahmed M., Hussain W. A., Tahir M. S. Development and Validation of New HPLC Method for Simultaneous Estimation of L-Lysine Hydrochloride and L-Carnitine-L-Tartrate in Pharmaceutical Dosage Form // Indian J. Pharm. Sci. – 2015. – V. 77 (4). – P. 434–438. https://doi.org/10.4103/0250-474X.164772.
9. Laura S. H., Maria C. P., Carmen G. R., Antonio L. C., Maria L. M. Determination of L-and D carnitine in dietary food supplements using capillary electrophoresis tandem mass spectrometry // Food Chem.– 2010. – V. 120. – P. 921–928. https://doi.org/10.1016/j.foodchem.2009.11.004.
Published
2019-03-18
How to Cite
Voitiuk, O. D., Yegorova, A. V., Scrypynets, Y. V., KashutskуyS. N., Kluchnik, O. G., & Umetskaya, I. V. (2019). HPLC-determination of active components in dietary supplement «L-CARNITINE smart». Farmatsevtychnyi Zhurnal, (1), 86-96. https://doi.org/10.32352/0367-3057.1.19.08
Section
Production, quality control, standardization of medicines