The qualitative composition and quantitative content of flavonoids in the aerial part of the speciesbelonging to subfamily Nepetoideae family Lamiaceae

  • M. I. Shanayda Horbachevsky Ternopil State Medical University
Keywords: Lamiaceae,Ocimum americanum, Lophanthus anisatus, Satureja hortensis, flavonoids


Eessential oil herbs have long been used in medicine as anti-inflammatory, antispasmodic, antibacterial, expectorant and sedative drugs. At present phenolic compounds including flavonoids, play an important role in the total biological activity of essential oilplants.

The aim of this research – the identification and analysis of flavonoids in the aerial parts of three species belonging to the subfamily Nepetoideae family Lamiaceae: Ocimum americanum, Lophanthus anisatus and Satureja hortensis. It was identified the flavonoids by used qualitative reactions and chromatographic methods: paper, thin-layer and high performance liquid chromatography.

It was detected the presence of a number of aglycone and glycoside flavonoids. It was found  the flavonol (rutin, isoquercitrin and hyperoside) and flavone (apigenin, luteolin and their derivatives) compounds. Lophanthus anisatus is characterized the highest diversity of flavonoids. Ir was revealed the presence of isoquercitrinand luteolin derivatives in all investigated plants; other compounds was detected selectively.

Lophanthus anisatus herb set the maximum content of apigenin and its derivatives ((30,71·10-2% и 26,79·10-2%, respectively); the highest content of rutin was found in the grass Ocimum americanum (62,06·10 2%) and Satureja hortensis (28,56·10-2%).

The results of investigations can be used in chemotaxonomy of the subfamily Nepetoideae and further planning of pharmacological studies.


1. Popova NV, Dichtyarev SI, Maslova NF, Litvinenko V. I. Antibiotic properties of luteolin // Ukr. biopharm journ - 2011. - No. 6 (17). - pp. 4-11.

2. Koshovy O. M. Phenolic composition of some representatives of the subgenus Sclarea genus Salvia // Act. pit pharmacy and honey science and practice. - 2012. - No. 3 (10). - P. 11-14.

3. Kurkina A. V. Resource-saving aspects of research of raw materials of essential oil medicinal plants containing flavonoids // Izv. Samara Scientific Center of the Russian Academy of Sciences. - 2011. - T. 13, No. 1 (4). - P. 853-856.

4. Marchishin SM, Shanaida M.I. Pigment composition of the above-ground part of Monarda fistulosa L. // Act. pit pharmacy and honey science and practice. - 2012. - No. 2 (9). - P. 31-32.

5. Yarosh A., Shalamay A., Bobkov V., Nikolaeva A. Natural flavonides as promising drugs / / Vishn. farm and farm - 2003. - No. 11. - P. 18-26.

6. Chumakova VV, Popova OI I. Lofant anisive (Agastache foeniculum L.) - a promising source for the production of medicines // Pharmacology and Pharmacology. - 2013. - № 1. - P. 41-46.

7. Shanaida M.I. Comparative analysis of phenolic compounds of some species of the family Lamiaceae Juss.// Tez. doc. scientific practice. conf. "Biologically active substances: fundamental and applied questions of obtaining and applying", May 23-28, 2011, Novy Svet, Ukraine. - P. 341.

8. Libus O.K., Rostyagov V.D., Kutko S.P., Khlypenko L. A. Ethereal and pine-aromatic plants. - Kherson: Aylant, 2004. - 272 p.

9. Gohari A., Saeidnia S., Gohari M. et al. Bioactive flavonoids from Satureja atropatana Bonge. // Nat. Prod. Res - 2009. - V. 23 (17). - P. 1609-1614.

10. Reshma K., Ashalatha R., Dinesh M., Vasudevan D. Effect of Ocimum flavonoids as a radioprotector on erythrocyte antioxidants in oral cancer // Indian J. Clin. Biochem - 2005 - 20 (1). - P. 160-164.

11. Jianxiong Y., Guo J., Yuan J. In vitro antioxidant properties of rutin // Food Sci. Technol - 2008. - V. 41 (1). - R. 1060-1066.

12. Staszek D., Orłowska M., Waksmundzka-Hajnos M. et al. Marker fingerprints derived from TLS and HPLC for selected plants from the Lamiaceae family // J. Liquid Chromatography. - 2013. - V. 36 (17). - P. 2463-2475.
How to Cite
Shanayda, M. I. (2018). The qualitative composition and quantitative content of flavonoids in the aerial part of the speciesbelonging to subfamily Nepetoideae family Lamiaceae. Farmatsevtychnyi Zhurnal, (4), 71-76. Retrieved from
Pharmacological, phytochemical research