Quantitative determination of the content of phenolic compounds in a gel with the propolis phenolic hydrophobic drug and azelaic acid
Abstract
Recently there has been an increased interest in creation of medicines based on apiculture products. It is known that the composition of the standardized substance of the propolis phenolic hydrophobic drug contains flavones, flavonols, phenolcarboxylic acids and oxycoumarines with a quantitative predominance of flavonols and flavones, which give the antimicrobial, anti-inflammatory and other biological activities to this substance in the absence of any toxic action on a living organism.
The composition of new extemporaneous formulations in the form of a gel with propolis phenolic hydrophobic drug as the main active pharmaceutical ingredient for treating acne has been developed.
The aim of the current study was to develop the express methods for quantitative determination of the total content of phenolic compounds in the antimicrobial drug Propolis in the form of a gel containing azelaic acid in combination with propolis phenolic hydrophobic drug by spectrophotometry.
It has been proposed to determine the total content of phenolic compounds in the drug by light absorption of ethanol solution at 290 ± 2 nm in a cell with the layer thickness of 10 mm using 96% ethanol R as a compensation solution.
The method has been developed and the possibility of quantitative determination of the total content of phenolic compounds in the antimicrobial drug containing AA in combination with propolis phenolic hydrophobic drug has been shown by the method of absorption spectrophotometry in the UV region of the spectrum using the reference solution of potassium dichromate in sulphuric acid R. The content of phenolic compounds in 1 g of the gel has been determined, it is 0.0265 g (RSD = 2.2%).
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