Efficacy decametoxin in vitro for quick inactivation of respiratory coronavirus
Abstract
Despite the fact that specific prophylaxis agents have already been widely introduced into medical practice in all countries of the world, and antiviral drugs are being developed and are undergoing the first stages of clinical trials, SARS-CoV-2 continues to spread in the human population. In this regard, an urgent medical problem today is the expansion of the arsenal of effective disinfectants and antiseptics, the action of which would be aimed at the rapid and complete inactivation of extracellular coronavirus, which is a very important element in controlling the spread of COVID-19.
The aim of the study was to evaluate the ability of decamethoxin to have a virucidal effect against SARS-COV-2 and other human coronaviruses on the model of respiratory coronavirus IBV (infectious bronchitis virus) with an exposure time of 30, 60 and 120 seconds.
Classical and modern virological research methods were used in the work: determination of the cytotoxic effect of decamethoxin in cell culture by the effect on their viability, cultivation, accumulation and determination of the infectious titer of IBV by cytopathic action in cell culture; assessment of the virucidal effect of decamethoxin by the suspension method to determine the residual infectious titer of the virus in cell culture by the method of limiting dilutions.
The effectiveness of the antiseptic decamethoxin from the group of quaternary ammonium compounds was studied in relation to the prototype strain of the IBV (infection bronchitis virus) coronavirus family in vitro. It has been established that an isotonic solution of decamethoxin at a concentration of 100 μg/ml completely inactivates 3.0 lg(TCD50/0.1 ml) of the prototype respiratory coronavirus strain with a clinically significant contact time of 30–120 seconds at room temperature(18–24 оС). Decamethoxine has been shown to be an effective, fast-acting antiseptic capable of completely inactivating a prototype coronavirus strain. The revealed virucidal properties of decamethoxine in pharmacopoeially significant concentrations in relation to coronavirus allow to recommend it as an antiseptic in the development of methods for non-specific prevention of coronavirus infection in humans.
References
Bontempi E. The Europesecondwave of COVID-19 infectionand the Italy «strange» situation // Environ. Res. – 2021. – V. 193. – P. 110476. https://doi.org/10.1016/j.envres.2020.110476
Zaliska O. M., Semenov O. M., Maksymovych N. M., Zabolotnia Z. O. ta in. Analiz tendentsii spozhyvannia antybakterialnykh ta protyvirusnykh likarskykh zasobiv v aptechnykh zakladakh pid chas pandemii COVID-19 v Ukraini // Farmats. zhurn. – 2021. – T. 76, № 4. – S. 43–54.
Zaliska O. M., Semenov O. M., Maksymovych N. M., Slabyi M. V. ta in. Doslidzhennia roli provizora u zabezpechenni karantynnykh zakhodiv pid chas pandemii COVID-19 // Farmats. zhurn. – 2020. – T. 75, № 6. – S. 16–25. https://doi.org/10.32352/0367-3057.6.20.02
Kristjanpoller W., Michell K., Minutolo M. C. A causal framework to determine the effectiveness of dynamic quarantine policy to mitigate COVID-19 // Appl. Soft Computing. – 2021. – V. 104. ‒ P. 107241. https://doi.org/10.1016/j.asoc.2021.107241
Kanakaraju D., Glass B. D., Vincent M. Disinfectants and coronavirus disease 2019 (COVID-19): A minireview// J. Sustainability Science and Management. – 2021. – V. 16, N 1. – P. 97‒102. https://doi.org/10.46754/jssm.2021.01.009
United States Environmental Protection Agency. List N: Disinfectants for Use Against SARS-CoV-2. ‒ URL: https://www.epa.gov/pesticide-registration/list-n-disinfectants-use-against-sars-cov-2
VAH List of Disinfectants. ‒ URL: https://vah-liste.mhp-verlag.de/en/
Kampf G., Todt D., Pfaender S., Steinmann E. Persistence of coronavirus eson inanimate surfaces and their inactivation with biocidal agents // J. Hosp. Infect. – 2020. ‒ V. 104, N 3. ‒ P. 246–251. https://doi.org/10.1016/j.jhin.2020.01.022
Lai A., Bergna A., Acciarri C. et al. Early phylogenetic estimate of the effective reproduction number of SARS-CoV-2 // J. Med. Virol. – 2020. – V. 92. ‒ P. 675–679. https://doi.org/10.1002/jmv.25723
Woodab A., Payneab D. The action of three antiseptics/disinfectants agains tenveloped and non-enveloped viruses // J. Hosp. Infect. ‒ 1998. ‒ V. 38, N 4. ‒ P. 283‒295. https://doi.org/10.1016/S0195-6701(98)90077-9
Kovalchuk V. P. ta in. Rezultaty eksperymentalnoho i klinichnoho doslidzhennia efektyvnosti antyseptychnoho preparatu dekasanu // Visn. Vinnytskoho derzh. un-tu. – 2002. – № 2. – S. 292‒294.
Panchuk S. I., Humeniuk M. I., Trokhymenko O. P., Dziublyk I. V. Virulitsydna diia dekametoksynu po vidnoshenniu do virusnykh tryheriv infektsiinoho zahostrennia bronkhialnoi astmy // Ukr. pulmonol. zhurn. – 2014. – № 2. – S. 48‒51.
Trokhymenko O. P., Panchuk S. I., Humeniuk M. I., Dziublyk I. V. Vyznachennia in vitro virulitsydnoi dii dekametoksynu na modeliakh prostykh i skladnykh virusiv yak mozhlyvykh tryheriv infektsiinoho zahostrennia bronkhialnoi astmy // Profilaktychna medytsyna: epidemiolohiia, mikrobiolohiia, virusolohiia, parazytolohiia ta infektsiini khvoroby. – 2013. – № 3‒4 (21). – S. 78‒84.
Dziublyk O. Ya., Dziublyk I. V., Trokhymenko O. P., Bororova O. L. Virulitsydna diia dekametoksynu in vitro po vidnoshenniu do koronavirusu infektsiinioho bronkhitu // Ukr. pulmonol. zhurn. – 2020. – № 2. – S. 27‒30. https://doi.org/10.31215/2306-4927-2020-108-2-27-30
Instruktsiia dlia medychnoho zastosuvannia likarskoho zasobu DEKASAN® (DESASANUM). ‒ URL: https://likicontrol.com.ua/%D1%96%D0%BD%D1%81%D1%82%D1%80%D1%83%-D0%BA%D1%86%D1%96%D1%8F/?%5B24656%5D
Dziublyk I. V., Trokhymenko O. P, Soloviov S. O. Kultura klityn u medychnii virusolohii. Navchalno-metodychnyi posibnyk. – Kyiv, 2015. – 144 s. ISBN 978-966-2696-98-1.
Humeniuk M. I., Humeniuk H. L., Opimakh S. H. Efektyvnist dekametoksynu proty skladnykh virusiv, nezalezhno vid yikh antyhennoi budovy: perspektyvy vykorystannia pry suchasnykh virusnykh zakhvoriuvanniakh dykhalnykh shliakhiv / Aktualnaia ynfektolohyia. ‒ 2020. ‒ URL: http://ai.zaslavsky.com.ua/article/view/196168/197747
Cell Proliferation Kit I (MTT). ‒ URL: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents--/117/135/11465007001bul.pdf
Skarha-Bandurova I. S., Feihina D. I. Zastosuvannia metodu lohistychnoi rehresii v medychnykh doslidzhenniakh: dys. Sumskyi derzh. un-tet. – 2014. ‒ URL: https://essuir.sumdu.edu.ua/bitstream-download/123456789/39121/1/Skarga-Bandurova_logistic%20regression.pdf 11
Vasylenko O. A., Sencha I. A. Matematychno-statystychni metody analizu v prykladnykh doslidzhenniakh. Navch. posibnyk. – 2012. ‒ URL: http://ir.nmapo.edu.ua:8080/bitstream/lib/284/1/Математично-статистичні%20методи%20аналізу%20в%20прикладни.pdf
Shcherbinska A. M., Diachenko N. S., Rybalko S. L., Nosach L. M. ta in. Vyvchennia antyvirusnoi dii potentsiinykh likarskykh zasobiv. Metod. rekomendatsii / Derzhavnyi farkolohichnyi tsentr MOZ Ukrainy. – Kyiv, 2000. – 40 s.
Sattar S. A.; Springthorpe V. S.; Karim Y.; Loro P. Chemical disinfection of non-porous inanimate surfaces experimentally contaminated with four human pathogenic viruses // Epidemiol. Infect. ‒ 1989. – V. 102. ‒ P. 493–505. https://doi.org/10.1017/S0950268800030211
Tessema B., Frank S., Bidra A. SARS-CoV-2 viral inactivation using low dose povidone-iodine oral rinse ‒ immediate application for the prosthodontic practic // J. Prosthodont. ‒ 2020. – V. 29, N 6. ‒ P. 459. https://doi.org/10.1111/jopr.13207
Parhar H. S., Tasche K., Brody R. M. et al. Topical preparations to reduce SARS-CoV-2 aerosol ization in head and neck mucosal surgery // Head. Neck. ‒ 2020. – V. 42, N 6. – P. 1268‒1272. https://doi.org/10.1002/hed.26200
Challacombe S. J., Kirk-Bayley J., Sunkaraneni V. S., Combes J. Povidone iodine // Br. Dent. J. – 2020. – V. 228, N 9. – P. 656‒657. https://doi.org/10.1038/s41415-020-1589-4
Samantha F., Seth M. Brown, Joseph A. Capriotti et al. In Vitro Efficacy of a Povidone-Iodine Nasal Antiseptic for Rapid Inactivation of SARS-CoV-2 // JAMA Otolaryngol. Head. Neck. Surg. ‒ 2020. – V. 146, N 11. – P. 1054‒1058. https://doi.org/10.1001/jamaoto.2020.3053
Gorbalenya A. E., Baker S. C., Baric R. S. et al. The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and namingit SARS-CoV-2 // Nat. Microbiol. – 2020. – V. 5. – P. 536–544. https://doi.org/10.1038/s41564-020-0695-z
Cassandra L. Schrank, Kevin P. C. Minbiole, William M. Wuest. Are Quaternary Ammonium Compounds, the Workhorse Disinfectants, Effective against Severe Acute Respiratory Syndrome-Coronavirus-2? // ACS Infect. Dis. – 2020. ‒ V. 6, N 7. ‒ P. 1553–1557. https://doi.org/10.1021/acsinfecdis.0c00265. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233851/
This work is licensed under a Creative Commons Attribution 4.0 International License.
