Studing conditions of sildenafil extraction by organic solvents
Abstract
The dependence of sildenafil extraction efficiency from pH value and nature of organic solvents was studied. Maximal amounts of this preparation are extracted by 1,2-dichlorethane at pH 6,0—8,0.
The influence of electrolytes on sildenafil extraction efficiency was studied. Electrolytes solution of natrium chloride (5% and 25 %) and solution of ammonium sulfate (5 %, 25 % and 50 %) decrees extraction efficiency of this preparation.
The extraction-photometric method of quantitative determination of sildenafil was developed. This method is based on formation of complex with bromocresol green in buffer at pH 4,5. The limit of determination of this method is 8 mg in 15 ml of sample volume. Relative error of this method is ± 1,05 %.
References
Baselt R.C. Disposition of toxic drugs and chemicals in man. - Foster City, CA: Biomedical Publications, 202. - 142 p.
Cooper J.D., Muirhead D.C., Taylor J.E. et al. //.. Chromatogr. - B. Biomed. Sei. - Appl. 1997. Vol. 701. P. 87-95.
Dumestre-Toulet V., Cirimele V., Gromb S. et al. // Forensis Sci. Int. - 2002. - Vol. 126. P. 71-76.
Fraunfelder F. T., Loties A.M. // J. Toxicol. - Cut & Ocular Toxicol. - 2000. - Vol. 19. - P. 21-25.
Johnson R D., Lewis R.J., Canfield D.V. et al. // J. Chromatogr. B Analyt. Technol. Life Sci. - 2004. - Vol. 805. P. 223-34.
Cloner R.A. // Am. J. Cardiol. - 2000. - Vol. 86, 1s. 2. - P. 57-61.
Sienel R.L. // Hum. Exp. Toxicol. - 2003. - Vol. 22, 1s. 8. - P. 463-464.
Sninlapawittayatorn K., Chattipakorn S., Chattipakorn N. // Braz. J. Med. Biol. Res. - 2005. - Vol. 38, Is. 9. - P. 1303-1311.
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