The study of lipotropic action of extract from fruit prunus domestica «Prunofit» byanimal models of alcoholic liver diseases

Keywords: lipotropic action, methionine, total lipids, alcoholic hepatitis, fruits of Prunus domestica

Abstract

Searchingof drugs that normalize the function of the digestive glands are important because disruption of digestion underlie the pathogenesis of many diseases such as gastroesophageal reflux disease, hepatitis, gastric ulcer, irritable bowel syndrome, cancer etc. The use of herbal objects containing fibres is a promising direction for solving of the mentioned problem. We were attracted by fruits of Prunus domestica which are rich in fibres (homo- and heteropolsaccharides) and are used in folk medicine as a laxative and hepatoprotective agent.

The aim of this experimental study was to investigate the lipotropic properties of «Prunofit» extract. The study of lipotropic properties of the «Prunofit» was carried out in the conditions of subacute toxic liver damage caused by the introduction of ethanol.The content of total lipids (TL), total cholesterol (TCh), triacylglycerols (TGs), unsaturated fatty acids (UFA) and total phospholipids (TPL) were determined in liver and blood serum.

Obtained results of the lipotropic properties study of the «Prunofit» extract at a dose of 200 mg/kg against the background of alcoholic liver damage showed a decrease in the intensity of lipolysis, fatty hepatosis, and manifestations of hyperlipidemia. It has occurred due to a decrease in the content of total lipids, cholesterol, triglycerides and free fatty acids in the homogenate rat liver by 27.9%, 7.2%, 27.5% and 38%, respectively, and rat serum by 28%, 42.2%, 8.15%, 47.1%, respectively, compared with the control pathology. Against the background of model pathology, «Prunofit» extract tended to increase the content of total phospholipids in the liver homogenate by 37.34% and in serum by 30.2% compared with the control pathology. According to its ability to inhibit fatty liver infiltration, the «Prunofit» extract was at the level of the reference drug «Methionine» at a dose of 155 mg/kg.

References

Wu Т., Rayner С. К., Young R. L., Horowitz M. Gut motility and enteroendocrine secretion // Curr. Opin. Pharmacol. – 2013. – V. 13, N 6. – P. 928–934.

Drinnan M., Powell J., Nikkar-Esfahani A., Heading R. C., Doyle J. Gastroesophageal and extraesophageal reflux symptoms: Similarities and differences // Laryngoscope. – 2014. – N 30.

Drossman D. A., Hasler W. L. Rome IV – Functional GI Disorders: Disordersof Gut-Brain Interaction //Gastroenterol. – 2016. – V. 150, N 6. – P. 1257–1261.

American Gastroenterological Association Medical Position Statement on Constipation // Gastroenterol. – 2013. – N 144. – P. 211–217. https://doi.org/10.1053/j.gastro.2012.10.029

Quigley E. M. Prucalopride: safety, efficacy and potential applications. Ther. Adv. // Gastroenterol. – 2012. – V. 5, N 1. – P. 23–30.

Patent na vynaxid № C2 118602. Sposib oderzhannya vodorozchynnogo polisaxarydnogo kompleksu z poslablyuyuchoyu aktyvnistyu z plodiv slyvy domashnoyi / Komisarenko A. M., Upyr T. V., Senyuk I. V. ta in. – Zayavl. 06. 03. 2017; Opubl. 11. 02. 2019, Byul. № 3.

Anroop B. Nair, Shery Jacob. A simple practice guide for dose conversion between animals and human // J. Basic. Clin. Pharm. – 2016. – V. 7, N 2. – P. 27–31. https://doi.org/10.4103/0976-0105.177703

Doklinicheskie issledovaniya lekarstvennykh sredstv: metod. rek. / Pod red. A. V. Stefanova. – K: Avitsenna, 2002. – 528 s.

Orel N. M. Biokhimiya membran: metod. posobie k lab.zanyatiyam dlya stud. biol. f-ta. – Minsk: BGU, 2010. – 28 s.

Prokhorova M. I. Metody biokhimicheskikh issledovaniy (lipidnyy i energeticheskiy obmen): uch. posobie. – L.: Izd-vo Leningr. un-ta, 1982. – 272 s.

Evans U. G., Morre D. D. Biologicheskie membrany. Metody / Pod red. Findleya Dzh., Evansa U. G. – M: Mir, 1990. – 424 s.

Ježek J., Jabůrek M., Zelenka J., Ježek P. Mitochondrial phospholipase A2 activated by reactive oxygen species in heart mitochondria induces mild uncoupling // Physiol. Res. – 2010. – V. 59, N 5. – P. 737–747.

Published
2020-12-09
How to Cite
Kravchenko, V. M., Shovkova, Z. V., Senyuk, I. V., & Shovkova, O. V. (2020). The study of lipotropic action of extract from fruit prunus domestica «Prunofit» byanimal models of alcoholic liver diseases. Farmatsevtychnyi Zhurnal, (6), 86-91. https://doi.org/10.32352/0367-3057.6.20.09
Section
Pharmacological, phytochemical research