Determination of the sum of flavonoids in hops (Humulus L.) with the use of luminescent sensor on the basis of the complex of rutin with yttrium (III)

  • S. V. Beltyukova Odesa National Academy of Food Technologies
  • Ye. V. Cherednychenko Odesa National Academy of Food Technologies
  • О. I. Teslyuk Bogatsky Physico-chemical institute of the NAS of Ukraine, Odesa
Keywords: flavonoids, luminescence, yttrium, hops


Hop polyphenols are one of the main groups of biologically active agents defining medicinal properties of hops. Various chromatographic, electrochemical, сhromatographic methods of the analysis are used for the determination of flavonoids.

The aim of this work was to develop the method of quantitative determination of flavonoids in hops with the use of luminescent properties of complex compounds of flavonoids with ions of yttrium (III) and citrate-ion in the presence of the bovine serum albumin (BSA) as an analytical signal.

Rutin was used as the polyphenolic standard. Its luminescence intensity increases with a complex formation with ions of yttrium (III). It was shown that Ilum of yttrium (III)‒rutin complex considerably increased at the presence of the citrate-ions. Citrate-ion is coordinated by an ion of yttrium (III) on carboxyl and hydroxyl groups that leads to replacement of a molecule of water from the internal sphere of a complex and, as a result, to increase in intensity of luminescence. It was established that intensity of luminescence of the complex increased in presence of BSA. Nonradiative energy losses decreased owing to solubilization of rutin into the microphases of globular protein, as solubilization stipulated the protection of a fluorophore from suppression by water molecules. It was established that the maximum intensity of luminescence of the complex of yttrium with rutin and citrate-ions in the presence of BSA could be observed at the pH 6,5‒7,5. It was shown that ethanol solutions of flavonoids of hops interacted like routine with ions of yttrium (III), citrate-ions, their intensity increased in the presence of BSA. The optimum conditions for receiving an analytical signal of the proposed luminescent sensor were found.

The method of luminescent determination of the sum of flavonoids in the granulated hops has been developed. The received results were verified by the method of spiked samples and spectrophotometric method with AlCl3.


1. Lyashenko M.I., Protsenko L.V. Prenylflavonoids of hops and beer / Agro-industrial production of Polissya: Sb. sciences works. - 2009. - No. 2. - P. 52-56.
2. Kartsova L. A., Alekseeva AV Chromatographic and electrophoretic methods for the determination of polyphenolic compounds, Zh. analyte chemistry - 2008. - Vol. 63, No. 11. - P. 1126-1136.
3. Vanidze M. R., Kalandia A. G., Shalashvili AG Flavonoid compounds of fayhoa fruits // Chemistry Rast. raw materials - 2009. - No. 3. - P. 103-108.
4. Temerdashev Z. A., Frolova NA, Kolychev IA Determination of phenolic compounds in medicinal plants by the method of reverse-phase HPLC // Journal. analyte chemistry - 2011. - T. 66, No. 4. - P. 417-424.
5. Dmitrienko VA, Kudrinskaya VA, Apary VV Methods for isolation, condensation and determination of quercetin // Ibid. - 2012. - T. 67, No. 4. - P. 340-353.
6. Ziyatdinova G.K., Budnikov G.K. Definition of flavonols in pharmaceutical products by the method of voltammetry // Khim.-farm. journ - 2005. - Vol. 39, No. 10. - P. 54-56.
7. Sorokina O. N., Sumina E. G., Petrakova AV, Barysheva S. V. Spectrophotometric determination of the total content of flavonoids in medicinal preparations of plant origin // Izvestiya Saratov Univ. Sir Chemistry, Biology, Ecology. - 2013. - Vol. 13, Vip. 3. - p. 8-11.
8. Lobanova AA, Budaeva VV, Sakovich GV Investigation of biologically active flavonoids in extracts from plant raw materials // Chemistry Rast. raw materials - 2004. - No. 1. - P. 47-52.
9. Beketov EV, Abramov AA, Nesterova OV, Kondrashev SV Identification and quantification of flavonoids in the fruits of cherry juice of the ordinary // Vestn. Moscow un-that Sir 2. Chemistry. - 2005. - Vol. 46, No. 4. - P. 259-262.
10. Alekseeva MA, Eller K. I., Arzamastsev AP. Determination of polyphenolic components of hops by means of reverse-phase HPLC // Chim.-farm. journ - 2004. - Vol. 38, No. 12. - P. 39-41.
11. Belikov VV Methods of analysis of flavonoid compounds // Pharmacia. - 1970. - No. 1. - P. 68.
How to Cite
Beltyukova, S. V., Cherednychenko, Y. V., & TeslyukО. I. (2018). Determination of the sum of flavonoids in hops (Humulus L.) with the use of luminescent sensor on the basis of the complex of rutin with yttrium (III). Farmatsevtychnyi Zhurnal, (3-4), 82-86. Retrieved from
Pharmacological, phytochemical research