Determination of the qualitative and quantitative content of hydroxycinnamic acids in Gentiana Cruciata L. herb
Gentiana cruciata L. is a herbaceous perennial flowering plant in the Gentianaceae family. This plant is widespread in the territory of Ukraine, namely the southern forest areas, forest-steppe and Crimea. Gentiana cruciata L. grows on dry meadows and slopes, among shrubs, young rare pine forests and along the edges.
From ancient times Gentiana cruciata L. was used in folk medicine as appetizing and stimulating the secretion of digestive glands agent; it increases the motility of digestive tract. Gentiana cruciata L. also has anti-inflammatory, antiseptic and anthelmintic properties. Pharmacological action of Gentiana cruciata L. is caused by the presence of biologically active substances, the content of which is insufficiently studied. And hydroxycinnamic acids are among these substances.
The object of our research is the Gentiana cruciata L. herb, collected at territory Volove, Ternopil region, during flowering period in 2014.
Identification of hydroxycinnamic acids in Gentiana cruciata L. herb was performed using iron (III) chloride. The greenish gray colour appeared and this tells about the presence of phenolic substances in our object.
Using paper chromatography ferrulic, rosmarinic, neochlorogenic and caffeic acids were firstly identified in Gentiana cruciata L.
Content of hydroxycinnamic acids in our object was analyzed using spectrophotometry and it was determined at 3,75 ± 0,01% in recalculation into rosmarinic acid.
Determination of qualitative and quantitative content of hydroxycinnamic acids in Gentiana cruciata L. was also performed using high-performance liquid chromatography. 4 hydroxycinnamic acids were identified and their content was determined at 10,58∙10-3% for ferrulic acid, 19,36∙10-3% for caffeic acid, 16,04∙10-3% for p-coumaric acid, and 51,28∙10-3% for rosmarinic acid. The highest content belongs to rosmarinic acid.
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